I. Ultrastructural analysis

• Elucidation of the ultrastructure of normal and diseased tissues
• Immunogold-histochemistry
• Double and triple localizations of proteins in tissues and cells
• Ultrastructural in situ hybridization
• Combined in situ hybridization and immunogold-histohemistry to detect the mRNA together with the corresponding protein
• Quantification of immunogold-histochemistry results

II. Histology

• Paraffinhistolology of normal and dieased tissues and cells
• Semithin sections
• Immunohistochemistry
• Non-radioactive in situ hybridization
• Immunfluorescence
• In situ-RT-PCR

III. Molecular biology

• Isolation of mRNA from native tissue, also from those rich in extracellular matrix, like cartilage and bone
• cDNA synthesis
• probe preparation for in situ hybridization via PCR
• PCR
• Quantitative real-time PCR
• Microarray
• siRNA
• Cell immortalization with hTERT or SV40
• CRISPR/cas9

IV. Cell biology

• Cell culture of primary human cells, for example chondrocytes in 3D alginate
• Western Blot
• Production of monoclonal antibodies
• FACS
• Protein overexpression in primary human cells